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Project 52 Injectable hydrogels for the delivery and differentiation of stem cells
Onderzoekslijn Tissue Engineering
Omschrijving Tissue engineering approaches involving injectable and degradable carriers for cell transplantation offer specific advantages over preformed scaffolding structures such as the possiblity of minimal invasive implantation, the ability to fill a desired shape and the facile incorporation of growth factors and other biologically active molecules.
Significant effort is directed towards the delivery and differentiation of stem cells embedded in hydrogels to engineer cartilage and bony tissues. A number of natural and synthetic polymers are currently being employed in the preparation of such injectable fomulations. Amongst there are alginates, collagen, fibrin, PEG-based hydrogels and oligo(poly(ethylene glycol) fumarate) hydrogels and porous poly(lactide) beads [1,2,3,4].
A suitable environment for tissue generation can be created by incorporating biologically active factors such as growth factors and differentiation factors in the scaffold. As succesful vascularization of the tissue engineered structure is essential for nutrient transport and integration, such biological activity should also be directed at promoting angiogenesis. In cell therapy, angiogenesis has been induced by taking advantage of the plasticity of adult stem cells. Tateishi-Yujarna showed that autologous transplantation of bone-marrow cells in patients with end stage chronic limb ischemia (CLI) resulted in a striking increase in number of visible collateral blood vessels [5]. An injectable, biodegradable hydrogel-based system in which these adult stem cells are incorporated would prevent or delay homing of the cells to the marrow and allow incorporation of (angiogenic) differentiation factors.
Components in the natural extra-cellular matrix (ECM) that play an important role in the functioning of the cellular microenvironment are collagen, adhesive proteins, growth factors, proteoglycans and glycosaminoglycans (GAGs). In this project, we will prepare an artificial ECM that consists of a resorbable PEG-based hydrogel with covalently bound heparin. Heparin-binding adhesive proteins, growth factors and other biologically active molecules will be incorporated into the gels via ionic interactions. This approach can be useful in cartilage and bone tissue engineering as well as in the formation of blood vessels. As the PEG-based hydrogel will contain aliphatic polyester and/or carbonate sequences, tunable degradation properties can be realized. The hydrophilic PEG and GAG components in the networks will result in high hydration of the gels. The viability, adhesion and growth and differentiation of adult stem cells embedded in these gels will be investigated.
Projectleider Prof.dr. I.(Istvan) Vermes
Instituut Medlon BV Locatie Medisch Spectrum Twente Enschede
Trefwoorden scaffold, PEG-based hydrogels, stem cells, angiogenesis, vasculogenesis
Status Lopend
Periode 1-2001 - 1-2009
Partners Prof. Dr. J. Feijen, University of Twente
Prof. dr. D.W. Grijpma, University of Groningen
Medewerkers Y. Song, PhD student
dr. A.A. Poot, hoofddocent University of Twente
M. Kamphuis, rearch technician
Financiering 1e Geldstroom - Intern

Dutch program for tissue engineering (DPTE)